Ultrahigh-Throughput Multiplexed Screening of Purified Protein from Cell-Free Expression Using Droplet Microfluidics
Anqi Chen, Wentao Xu, Xinge Zhang, Junming Lao, Xing Zhao, Karla Milčić, David A. Weitz
Abstract
Lipase A (BsLipA) for improved thermotolerance. We screen a combinatorial library where 15 residues are saturated and identify multiple variants with 9-12 substitutions and >40 °C improvement in thermotolerance after a single round of mutagenesis. This platform offers a robust, scalable solution for protein engineering.
Topics & Concepts
ChemistryMicrofluidicsThroughputMultiplexingProtein expressionHigh-throughput screeningNanotechnologyBiophysicsComputational biologyChromatographyBiochemistryTelecommunicationsMaterials scienceBiologyGeneComputer scienceWirelessInnovative Microfluidic and Catalytic Techniques Innovation3D Printing in Biomedical ResearchElectrowetting and Microfluidic Technologies