The Small Open Reading Frame‐Encoded Peptides: Advances in Methodologies and Functional Studies
Lei Chen, Ying Yang, Yuanliang Zhang, Kecheng Li, Hongmin Cai, Hongwei Wang, Qian Zhao
Abstract
Small open reading frames (sORFs) are an important class of genes with less than 100 codons. They were historically annotated as noncoding or even junk sequences. In recent years, accumulating evidence suggests that sORFs could encode a considerable number of polypeptides, many of which play important roles in both physiology and disease pathology. However, it has been technically challenging to directly detect sORF-encoded peptides (SEPs). Here, we discuss the latest advances in methodologies for identifying SEPs with mass spectrometry, as well as the progress on functional studies of SEPs.
Topics & Concepts
Open reading frameBiologyENCODEComputational biologyGeneTranslation (biology)GeneticsPeptide sequenceMessenger RNARNA and protein synthesis mechanismsMachine Learning in BioinformaticsAdvanced Proteomics Techniques and Applications