Molecular characterization of clinical carbapenem-resistant Enterobacterales from Qatar
Fatma Ben Abid, Clement K. M. Tsui, Yohei Doi, Anand Deshmukh, Christi L. McElheny, William C. Bachman, Erin L. Fowler, Ahmed Albishawi, Kamran Mushtaq, Emad Bashir Ibrahim, Sanjay H. Doiphode, Manal Hamed, Muna Almaslmani, Abdullatif Al‐Khal, Adeel A. Butt, Ali S. Omrani
Abstract
Abstract One hundred forty-nine carbapenem-resistant Enterobacterales from clinical samples obtained between April 2014 and November 2017 were subjected to whole genome sequencing and multi-locus sequence typing. Klebsiella pneumoniae (81, 54.4%) and Escherichia coli (38, 25.5%) were the most common species. Genes encoding metallo-β-lactamases were detected in 68 (45.8%) isolates, and OXA-48-like enzymes in 60 (40.3%). bla NDM-1 (45; 30.2%) and bla OXA-48 (29; 19.5%) were the most frequent. KPC-encoding genes were identified in 5 (3.6%) isolates. Most common sequence types were E. coli ST410 (8; 21.1%) and ST38 (7; 18.4%), and K. pneumoniae ST147 (13; 16%) and ST231 (7; 8.6%).