DeGlyPHER: An Ultrasensitive Method for the Analysis of Viral Spike <i>N</i> -Glycoforms
Sabyasachi Baboo, Jolene K. Diedrich, Salvador Martínez‐Bartolomé, Xiaoning Wang, Torben Schiffner, Bettina Gröschel, William R. Schief, James C. Paulson, John R. Yates
Abstract
Viruses can evade the host immune system by displaying numerous glycans on their surface “spike-proteins” that cover immune epitopes. We have developed an ultrasensitive “single-pot” method to assess glycan occupancy and the extent of glycan processing from high-mannose to complex forms at each N-glycosylation site. Though aimed at characterizing glycosylation of viral spike-proteins as potential vaccines, this method is applicable for the analysis of site-specific glycosylation of any glycoprotein.
Topics & Concepts
GlycanChemistryGlycosylationGlycoproteinEpitopeSpike (software development)Immune systemComputational biologyMannoseBiochemistryAntibodyImmunologyBiologyManagementEconomicsGlycosylation and Glycoproteins Researchvaccines and immunoinformatics approachesMonoclonal and Polyclonal Antibodies Research