Crucial gating residues govern the enhancement of peroxygenase activity in an engineered cytochrome P450 <i>O</i>-demethylase
Panxia Zhao, Yiping Jiang, Qian Wang, Jie Chen, Fuquan Yao, Zhiqi Cong
Abstract
The structurally crucial gating effect is elucidated for regulating the inflow of H 2 O 2 to enhance the peroxygenase activity of engineered CYP199A4 variants, which showed O -demethylation activity toward a variety of methoxy-substituted benzoic acids.
Topics & Concepts
GatingDemethylaseChemistryCytochrome P450CytochromeBiophysicsBiochemistryBiologyEnzymeGeneEpigeneticsPharmacogenetics and Drug MetabolismHistone Deacetylase Inhibitors ResearchMetal-Catalyzed Oxygenation Mechanisms