Actuated 3D microgels for single cell mechanobiology
Berna Özkale, Junzhe Lou, Ece Özelçi, Alberto Elósegui-Artola, Christina M. Tringides, Angelo S. Mao, Mahmut Selman Sakar, David Mooney
Abstract
up to a week. The mechanically active microgels are capable of generating up to 15% compressive strain and forces reaching 400 nN. As a proof of concept, we demonstrate the use of the mechanically active cell culture system in mechanobiology by subjecting singly encapsulated MSCs to optically generated isotropic compression and monitoring changes in intracellular calcium intensity.
Topics & Concepts
MechanobiologyMicrofluidicsMesenchymal stem cellMaterials scienceBiomaterialCalcium alginateNanotechnologyCellBiomedical engineeringIntracellularCell cultureSelf-healing hydrogelsBiophysicsChemistryCell biologyCalciumBiologyEngineeringGeneticsMetallurgyPolymer chemistryBiochemistry3D Printing in Biomedical ResearchCellular Mechanics and InteractionsMicrofluidic and Bio-sensing Technologies