Characterization of <i>bla</i> <sub>CTX-M-27</sub> /F1:A2:B20 Plasmids Harbored by Escherichia coli Sequence Type 131 Sublineage C1/ <i>H</i> 30R Isolates Spreading among Elderly Japanese in Nonacute-Care Settings
Nao Matsuo, Rina Nonogaki, Michiko Hayashi, Jun-ichi Wachino, Masahiro Suzuki, Yoshichika Arakawa, Kumiko Kawamura
Abstract
We characterized 29 bla CTX-M-27 -harboring plasmids of Escherichia coli sequence type 131 (ST131) sublineage C1/ H 30R isolates from healthy individuals and long-term-care facility (LTCF) residents. Most (27/29) plasmids were of the FIA, FIB, and FII multireplicon type with the same plasmid multilocus sequence typing (pMLST). Several plasmids (7/23) from LTCF residents harbored only bla CTX-M-27 as the resistance gene; however, their fundamental structures were very similar to those of previously isolated bla CTX-M-27 /F1:A2:B20 plasmids, suggesting their prevalence as a newly arising public health concern.
Topics & Concepts
PlasmidMultilocus sequence typingEscherichia coliBiologyGeneticsInsertion sequenceSequence analysisTypingSequence (biology)GeneEnterobacteriaceaeMicrobiologyMolecular biologyVirologyGenotypeGenomeTransposable elementAntibiotic Resistance in BacteriaPharmaceutical and Antibiotic Environmental ImpactsEnterobacteriaceae and Cronobacter Research