Comparison of RT-qPCR and RT-dPCR Platforms for the Trace Detection of SARS-CoV-2 RNA in Wastewater
Warish Ahmed, Wendy Smith, Suzanne Metcalfe, Greg Jackson, Phil M. Choi, Mary Morrison, Daniel J. Field, Pradip Gyawali, Aaron Bivins, Kyle Bibby, Stuart L. Simpson
Abstract
= 0.0067) samples. The positivity results also indicated that for the analysis of SARS-CoV-2 RNA in wastewater, including the eluate and pellet samples may further increase the detection sensitivity using RT-dPCR.
Topics & Concepts
Digital polymerase chain reactionReal-time polymerase chain reactionDetection limitSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)RNAWastewaterChromatographyMolecular biologyReverse transcription polymerase chain reactionChemistryCoronavirus disease 2019 (COVID-19)BiologyVirologyPolymerase chain reactionMessenger RNAMedicineEnvironmental scienceGeneBiochemistryEnvironmental engineeringPathologyDiseaseInfectious disease (medical specialty)SARS-CoV-2 detection and testingBiosensors and Analytical DetectionAdvanced biosensing and bioanalysis techniques