Process for an efficient lentiviral cell transduction
Anna Chiara Pirona, Risky Oktriani, Michael Boettcher, Jörg D. Hoheisel
Abstract
The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency of cell transduction. Low efficiencies with particular cell types and/or the high complexity of lentiviral libraries can cause insufficient representation. Here, we present a protocol that yielded substantial increases in transduction efficiency in various cell lines in comparison to several other procedures.
Topics & Concepts
Transduction (biophysics)CRISPRCellComputational biologyBiologySignal transductionCas9Cell biologyGenome editingGeneticsGeneBiochemistryCRISPR and Genetic EngineeringVirus-based gene therapy researchRNA Interference and Gene Delivery