Solid-phase capture and profiling of open chromatin by spatial ATAC
Enric Llorens-Bobadilla, Margherita Zamboni, Maja Marklund, Nayanika Bhalla, Xinsong Chen, Johan Hartman, Jonas Frisén, Patrik L. Ståhl
Abstract
Current methods for epigenomic profiling are limited in their ability to obtain genome-wide information with spatial resolution. We introduce spatial ATAC, a method that integrates transposase-accessible chromatin profiling in tissue sections with barcoded solid-phase capture to perform spatially resolved epigenomics. We show that spatial ATAC enables the discovery of the regulatory programs underlying spatial gene expression during mouse organogenesis, lineage differentiation and in human pathology.
Topics & Concepts
Profiling (computer programming)Computational biologyChromatinPhase (matter)BiologyChemistryComputer scienceGeneticsDNAOperating systemOrganic chemistryAdvanced biosensing and bioanalysis techniquesGenomics and Chromatin DynamicsAdvanced Biosensing Techniques and Applications