<sup>19</sup>F electron nuclear double resonance (ENDOR) spectroscopy for distance measurements using trityl spin labels in DNA duplexes
Nargiz B. Asanbaeva, Д. С. Новопашина, Olga Yu. Rogozhnikova, Victor M. Tormyshev, Annemarie Kehl, А. А. Суханов, Аndrey V. Shernyukov, Alexander M. Genaev, Alexander A. Lomzov, Marina Bennati, Andreas Meyer, Elena G. Bagryanskaya
Abstract
F and the trityl radical predicted from molecular dynamic (MD) simulations. Moreover, for some cases, ENDOR spectral simulations based on MD results were able to reproduce the conformational distribution reflected in the experimental ENDOR line broadening. Additionally, MD simulations provided more detailed information about the melting of terminal base pairs of the oligonucleotides and about the configuration of the trityls relative to a DNA end.
Topics & Concepts
SpectroscopyElectron paramagnetic resonanceNuclear magnetic resonanceChemistryNuclear magnetic resonance spectroscopySpin (aerodynamics)CrystallographyElectron nuclear double resonanceResonance (particle physics)DNAAtomic physicsPhysicsThermodynamicsBiochemistryQuantum mechanicsElectron Spin Resonance StudiesAdvanced NMR Techniques and ApplicationsLanthanide and Transition Metal Complexes