Integration of Gill and Intestinal Osmoregulatory Functions to Assess the Smoltification Window in Atlantic Salmon
Jonás I. Silva-Marrero, Floriana Lai, Sigurd O. Handeland, Cindy Pedrosa, Virginie Gélébart, Pablo Balseiro, Juan Fuentes, Ivar Rønnestad, Ana S. Gomes
Abstract
The transfer time of Atlantic salmon smolts from freshwater to seawater remains a challenge in aquaculture, with the “smolt window” referring to the optimal timeframe for seawater readiness. Our study monitored Atlantic salmon osmoregulatory adaptations during smoltification under continuous light (LL) and winter signal regime (6 weeks LD 12:12) followed by 6 or 8 weeks of constant light. Fish were subsequently reared in seawater for 8 weeks and subjected to a stress event of cyclic hypoxia at the conclusion of the trial. Significant differences in growth trajectories were observed between the LL and LD groups, with fish receiving the winter signal showing compensatory growth after seawater transfer. Gill Na+/K+-ATPase (NKA) activity, plasma ions, glucose, and cortisol levels confirmed the importance of the winter signal for seawater adaptation. Molecular markers, including nka isoforms, Na+-K+-2Cl− cotransporter (nkcc), cystic fibrosis transmembrane conductance regulator (cftr), and Na+/HCO3− cotransporter (nbc), showed distinct temporal expression patterns, particularly in gills and midgut. Notably, the LD group with delayed seawater transfer exhibited enhanced growth and improved hypo-osmoregulatory capacity. These findings underscore the advantages of a winter signal in smoltification and suggest that delaying seawater transfer for up to 8 weeks could be beneficial.