A Bioorthogonal Decaging Chemistry of <i>N</i>-Oxide and Silylborane for Prodrug Activation both <i>In Vitro</i> and <i>In Vivo</i>
Zhicheng Yan, Yiyao Pan, Guofeng Jiao, Mengyu Xu, Dongguang Fan, Ziwei Hu, Jiarui Wu, Tao Chen, Miao Liu, Xiaoguang Bao, Hengte Ke, Xingyue Ji
Abstract
Bioorthogonal decaging chemistry with both fast kinetics and high efficiency is highly demanded for in vivo applications but remains very sporadic. Herein, we describe a new bioorthogonal decaging chemistry between N -oxide and silylborane. A simple replacement of “C” in boronic acid with “Si” was able to substantially accelerate the N -oxide decaging kinetics by 10 6 fold ( k 2: up to 10 3 M –1 s –1 ). Moreover, a new N -oxide-masked self-immolative spacer was developed for the traceless release of various payloads upon clicking with silylborane with fast kinetics and high efficiency (>90%). Impressively, one such N -oxide-based self-assembled bioorthogonal nano-prodrug in combination with silylborane led to significantly enhanced tumor suppression effects as compared to the parent drug in a 4T1 mouse breast tumor model. In aggregate, this new bioorthogonal click-and-release chemistry is featured with fast kinetics and high efficiency and is perceived to find widespread applications in chemical biology and drug delivery.