Antibacterial activities of coumarin-3-carboxylic acid against Acidovorax citrulli
Fadi Zhu, Xin Fu, Huochun Ye, Haixin Ding, Liu-Shuang Gu, Jing Zhang, Yongxia Guo, Gang Feng
Abstract
Coumarin-3-carboxylic acid (3-CCA), previously screened from natural coumarins, was found to possess strong antibacterial activity against Acidovorax citrulli ( Ac ). In order to further evaluate the activity of this compound against plant bacterial pathogens and explore its potential value as a bactericidal lead compound, the activity of 3-CCA against 14 plant pathogenic bacteria in vitro and in vivo was tested. Results showed that 3-CCA exhibited strong in vitro activities against Ac, Ralstonia solanacearum, Xanthomonas axonopodis pv. manihotis, X. oryzae pv. oryzae , and Dickeya zeae with EC 50 values ranging from 26.64 μg/mL to 40.73 μg/mL. Pot experiment results showed that 3-CCA had powerful protective and curative effects against Ac . In addition, the protective efficiency of 3-CCA was almost equivalent to that of thiodiazole copper at the same concentration. The results of SEM and TEM observation and conductivity tests showed that 3-CCA disrupted the integrity of the cell membrane and inhibited polar flagella growth. Furthermore, 3-CCA resulted in reductions in motility and extracellular exopolysaccharide (EPS) production of Ac while inhibiting the biofilm formation of Ac . These findings indicate that 3-CCA could be a promising natural lead compound against plant bacterial pathogens to explore novel antibacterial agents.