Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds
Mitchell Clough, Anderson Chen, Seong-Wook Park, Allison M. Ahrens, Jeffrey N. Stirman, Spencer L. Smith, Jerry L. Chen
Abstract
Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.
Topics & Concepts
Mesoscopic physicsCalcium imagingNeuroscienceBrain functionTwo-photon excitation microscopyMesoscale meteorologyNeuroimagingPhotonComputer sciencePhysicsMeasure (data warehouse)Cortex (anatomy)Brain activity and meditationBiological systemOpticsBiologyMaterials scienceCalciumElectroencephalographyFluorescenceMetallurgyMeteorologyQuantum mechanicsDatabaseAdvanced Fluorescence Microscopy TechniquesNeural dynamics and brain functionCell Image Analysis Techniques