Simple and reliable detection of CRISPR-induced on-target effects by qgPCR and SNP genotyping
Isabel Weisheit, Joseph Kroeger, Rainer Malik, Benedikt Wefers, Peter Lichtner, Wolfgang Wurst, Martin Dichgans, Dominik Paquet
Topics & Concepts
CRISPRBiologyGenotypingSanger sequencingSNP genotypingComputational biologyGenome editingGeneticsAmpliconDNA microarrayGenomeLocus (genetics)DNA sequencingGenePolymerase chain reactionGenotypeGene expressionCRISPR and Genetic EngineeringPluripotent Stem Cells ResearchGenetics, Aging, and Longevity in Model Organisms