Highly bioresistant, hydrophilic and rigidly linked trityl-nitroxide biradicals for cellular high-field dynamic nuclear polarization
Ru Yao, David Beriashvili, Wenxiao Zhang, Shuai Li, Adil Safeer, Andrei Gurinov, Antal Rockenbauer, Yin Yang, Yuguang Song, Marc Baldus, Yangping Liu
Abstract
a rigid piperazine linker. EPR experiments in the presence of reducing agents such as ascorbate and in HeLa cell lysates demonstrate the reduction resistance of StaPol-1. High DNP enhancements seen in small molecules, proteins and cell lysates at 18.8 T confirm that StaPol-1 is an excellent PA for DNP ssNMR investigations of biomolecular systems at high magnetic fields in reductive environments.
Topics & Concepts
Nitroxide mediated radical polymerizationPolarization (electrochemistry)ChemistryNanotechnologyMaterials scienceCombinatorial chemistryOrganic chemistryPolymerPhysical chemistryRadical polymerizationCopolymerAdvanced NMR Techniques and ApplicationsElectron Spin Resonance StudiesSolid-state spectroscopy and crystallography