Smartphone DNA or RNA Sensing Using Semisynthetic Luciferase-Based Logic Device
Dalu Chang, Ki Tae Kim, Eric Lindberg, Nicolas Winssinger
Abstract
Detection of specific oligonucleotide sequences is central to numerous applications, and technologies amenable to point-of-care diagnostics or end users are needed. Here, we report a technology making use of a bioluminescent readout and smartphone quantification. The sensor is a semisynthetic luciferase (H-Luc-PNA conjugate) that is turned on by a strand-displacement reaction. We demonstrated sensing of three different microRNAs (miRs), as representative cancer biomarkers, and demonstrate the possibility to integrate an AND gate to sense two sequences simultaneously.
Topics & Concepts
LuciferaseOligonucleotideBioluminescenceConjugateComputational biologyDNAmicroRNABiosensorRNANanotechnologyMultiple displacement amplificationComputer scienceBiologyMaterials sciencePolymerase chain reactionBiochemistryGeneMathematicsTransfectionMathematical analysisDNA extractionAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionCRISPR and Genetic Engineering