Probe Confined Dynamic Mapping for G Protein-Coupled Receptor Allosteric Site Prediction
Antonella Ciancetta, Amandeep Kaur Gill, Tianyi Ding, Dmitry S. Karlov, George Chalhoub, Peter J. McCormick, Irina G. Tikhonova
Abstract
dopamine receptor, and subsequent mutagenesis confirmed the prediction. The protocol provides fast and efficient prediction of key amino acid residues surrounding allosteric sites in membrane proteins and facilitates the structure-based design of allosteric modulators.
Topics & Concepts
Allosteric regulationG protein-coupled receptorAllosteric enzymeChemistryAllosteric modulatorBiophysicsSmall moleculeReceptorBiochemistryBiologyReceptor Mechanisms and SignalingNeuropeptides and Animal PhysiologyMass Spectrometry Techniques and Applications