Mass Spectrometry for Assessing Protein–Nucleic Acid Interactions
Xiaomei He, Xingyuan Chen, Yinsheng Wang
Abstract
Protein-nucleic acid interactions, including the interactions of proteins with DNA, RNA, and DNA/RNA hybrid, often confer important biological consequences to targeted nucleic acids. 1 It has been extensively explored because protein-nucleic acid interactions play essential roles in regulating numerous biological processes in human cells, including DNA replication and transcription, DNA damage response, epigenetic modifications, RNA processing, and RNA translation, among others. 2,3Aberrant protein-nucleic acid interactions have deleterious effects on human health and result in many human diseases. 4hus, a comprehensive study of protein-nucleic acid interactions is vital for understanding the molecular mechanisms underlying these diseases and for providing an important knowledge basis for disease diagnosis and therapies.