Spatial multimodal analysis of transcriptomes and metabolomes in tissues
Marco Vicari, Reza Mirzazadeh, Anna Nilsson, Mohammadreza Shariatgorji, Patrik Bjärterot, Ludvig Larsson, Hower Lee, Mats Nilsson, Julia Foyer, Markus Ekvall, Paulo Czarnewski, Xiaoqun Zhang, Per Svenningsson, Lukas Käll, Per E. Andrén, Joakim Lundeberg
Abstract
We present a spatial omics approach that combines histology, mass spectrometry imaging and spatial transcriptomics to facilitate precise measurements of mRNA transcripts and low-molecular-weight metabolites across tissue regions. The workflow is compatible with commercially available Visium glass slides. We demonstrate the potential of our method using mouse and human brain samples in the context of dopamine and Parkinson's disease.
Topics & Concepts
TranscriptomeComputational biologyComputer scienceBiologyGeneticsGeneGene expressionSingle-cell and spatial transcriptomicsAdvanced Proteomics Techniques and ApplicationsCell Image Analysis Techniques