Luciferase-based GloSensor™ cAMP assay: Temperature optimization and application to cell-based kinetic studies
Fang I Wang, Gucci Ding, Garmen S Ng, S. Jeffrey Dixon, Peter Chidiac
Topics & Concepts
G protein-coupled receptorAllosteric regulationBiosensorLuciferaseIntracellularReceptorSecond messenger systemSignal transductionChemistryAdenosineTransfectionCyclic adenosine monophosphateCytosolCell biologyBiophysicsBiologyBiochemistryEnzymeGeneReceptor Mechanisms and SignalingPhosphodiesterase function and regulationAdenosine and Purinergic Signaling