A novel dual-responsive colorimetric/fluorescent probe for the detection of N2H4 and ClO− and its application in environmental analysis and bioimaging
Boyu Lv, Zhonglong Wang, Yisheng Wu, Yiming Zheng, Zhennan Cui, Jia Li, Wen Gu
Abstract
Hydrazine (N 2 H 4 ) and hypochlorite (ClO − ) are both reactive chemical substances extensively utilized across various industrial domains. Excessive hydrazine (N 2 H 4 ) and hypochlorite (ClO − ) can pose significant risks to the environment, ecosystems, and human health. In order to assess and control the environmental hazard caused by N 2 H 4 and ClO − , there is an imperative need for efficient methods capable of rapid and precise detection of these contaminants. This paper introduces a novel dual-responsive colorimetric/fluorescent probe (MDT) for the detection of N 2 H 4 and ClO − in environmental and biological samples. The probe exhibits turn-on fluorescent responses to N 2 H 4 or ClO − with low detection limits (N 2 H 4 : 8 nM; ClO − : 15 nM), large Stokes shifts (N 2 H 4 : 175 nm; ClO − : 203 nm), short response time (N 2 H 4 : 4 min; ClO − : 5 s) and broad pH range (5−10). In practical applications, MDT has been successfully employed in detecting N 2 H 4 and ClO − in water and soil samples from diverse locations. Test strips loaded with MDT offer a visual and convenient means to track N 2 H 4 vapor and quantify N 2 H 4 and ClO − concentrations in solutions. Finally, MDT has been utilized for sensing N 2 H 4 and ClO − in Arabidopsis thaliana roots and living zebrafish. This study presents a promising tool for monitoring N 2 H 4 and ClO − in the environment and living organisms.