Sensitive electrochemiluminescence analysis of lung cancer marker miRNA-21 based on RAFT signal amplification
Qingyu Wang, Shuaibing Yu, Lianshun Zhang, Lei Wang, Jinming Kong, Lianzhi Li, Xueji Zhang
Abstract
An electrochemiluminescence approach based on surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) was developed for miRNA-21 detection for the first time. The SI-RAFT polymerization generates polymer chains with functional groups that are used to recruit luminol, enabling strong ECL signal output with low concentrations of miRNA-21, and greatly improving the detection sensitivity.
Topics & Concepts
ElectrochemiluminescenceRaftChain transferReversible addition−fragmentation chain-transfer polymerizationSensitivity (control systems)microRNALuminolPolymerizationChemistryFragmentation (computing)Materials sciencePolymerRadical polymerizationComputer scienceChemiluminescenceDetection limitChromatographyBiochemistryElectronic engineeringOrganic chemistryOperating systemGeneEngineeringAdvanced biosensing and bioanalysis techniquesMicroRNA in disease regulationRNA Interference and Gene Delivery