Critical Domains in the Specific Binding of Radiolabeled Vip3Af Insecticidal Protein to Brush Border Membrane Vesicles from <i>Spodoptera</i> spp. and Cultured Insect Cells
Yudong Quan, Maria Lázaro-Berenguer, Patricia Hernández-Martínez, Juan Ferré
Abstract
This study shows that Vip3Af binding sites are fully shared with Vip3Aa, only partially shared with Vip3Ca, and not shared with Cry1Ac and Cry1F in two Spodoptera spp. Truncated versions of Vip3Af revealed that only domains I to III were necessary for the specific binding, most likely because they can form the functional tetrameric oligomer and because domain III is supposed to contain the binding epitopes.
Topics & Concepts
SpodopteraBacillus thuringiensisBiologySf9BiochemistryHeterologousCry1AcBrush borderBinding siteCell biologyTrypsinPlasma protein bindingBinding proteinHeterologous expressionExiguaMembrane proteinBinding domainAlanineProtein domainVesicleTransport proteinPeptide sequenceInsectAmino acidCell membraneLigand binding assayDNA-binding proteinNuclear Structure and FunctionInsect Resistance and GeneticsViral Infectious Diseases and Gene Expression in Insects