miR‑181a‑5p inhibits the proliferation and invasion of drug‑resistant glioblastoma cells by targeting F‑box protein 11 expression
Xueyan Wen, Songrong Li, Mengchan Guo, Hongzhan Liao, Yongmin Chen, Xi Kuang, Xiao‐Ping Liao, Lin Ma, Qifu Li
Abstract
Glioblastoma (GBM) is the most common malignant primary tumor in the human central nervous system. The present study aimed to explore the molecular mechanism by which microRNA (miR)‑181a‑5p targets the F‑box protein 11 (FBXO11) in glioma cells to inhibit cell proliferation and invasion. Reverse transcription‑quantitative (RT‑q)PCR was performed to detect the expression levels of miR‑181a‑5p in U251TR cells, U251 cells, primary GBM tissues and relapsed GBM tissues in order to determine the association between miR‑181a‑5p and the chemoresistance of GBM cells. The expression levels of miR‑181a‑5p in GBM cells were modulated via transfecting miR‑181a‑5p mimics and inhibitors. Cell Counting Kit‑8 assays were undertaken to assess the effects of miR‑181a‑5p on drug sensitivity and proliferation of GBM cells. Wound healing assays were performed to examine the effects of miR‑181a‑5p on the migratory ability of GBM cells. Furthermore, the effects of miR‑181a‑5p on the invasive ability of GBM cells were analyzed using an <em>in vitro</em> invasion assay. Flow cytometry analysis was carried out to determine whether overexpression of miR‑181a‑5p can promote the apoptotic rate of GBM cells. RT‑qPCR and western blotting were employed to detect the effects of miR‑181a‑5p on mRNA and protein expression of FBX011. miR‑181a‑5p exhibited low expression in resistant GBM cell lines and recurrent tumor tissues. Dual‑luciferase reporter assays were utilized to detect luciferase activity to verify the targeted regulatory association between miR‑181a‑5p and FBXO11. Upregulation of miR‑181a‑5p promoted the sensitivity of GBM cells to temozolomide (TMZ), increased the apoptotic rate of GBM cells and significantly inhibited the invasive and migratory capacities of GBM cells. In drug‑resistant glioma cells, compared with the miR‑negative control group and the blank group, the expression of miR‑181a‑5p was significantly upregulated (P<0.01), while the expression of FBXO11 protein was downregulated. miR‑181a‑5p increased the sensitivity of GBM cells to TMZ. miR‑181a‑5p significantly inhibited the migratory and invasive capacities of GBM cells. miR‑181a‑5p may become a novel effective target for the treatment of GBM. The results of dual‑luciferase reporter assays indicated that miR‑181a‑5p could target the 3'‑untranslated region of FBXO11. The underlying mechanism may be targeted inhibition of <em>FBXO11</em> gene expression, or may be associated with apoptosis.