Long Noncoding RNA NRAV Promotes Respiratory Syncytial Virus Replication by Targeting the MicroRNA miR-509-3p/Rab5c Axis To Regulate Vesicle Transportation
Jian Li, Miao Li, Xiuli Wang, Mengfei Sun, Cuiqing Ma, Wenzhang Liang, Xue Gao, Lin Wei
Abstract
The mechanism of interaction between RSV and host noncoding RNAs is not fully understood. In this study, we found that the expression of long noncoding RNA (lncRNA) negative regulator of antiviral response (NRAV) was reduced in RSV-infected patients, and overexpression of NRAV facilitated RSV production in vitro , suggesting that the reduction of NRAV in RSV infection was part of the host antiviral response. We also found that NRAV competed with vesicle protein Rab5c for microRNA miR509-3p in cytoplasm to promote RSV vesicle transport and accelerate RSV proliferation, thereby improving our understanding of the pathogenic mechanism of RSV infection.
Topics & Concepts
BiologymicroRNAVirologyReplication (statistics)RNAViral replicationRespiratory systemVirusCell biologyGeneGeneticsAnatomyCancer-related molecular mechanisms researchRespiratory viral infections researchRNA modifications and cancer