MasterOfPores: A Workflow for the Analysis of Oxford Nanopore Direct RNA Sequencing Datasets
Luca Cozzuto, Huanle Liu, Leszek P. Pryszcz, Antonio Hermoso, Anna Delgado-Tejedor, Julia Ponomarenko, Eva Maria Novoa
Abstract
workflow can be executed on any Unix-compatible OS on a computer, cluster or cloud without the need of installing any additional software or dependencies, and is freely available in Github (https://github.com/biocorecrg/master_of_pores). This workflow simplifies direct RNA sequencing data analyses, facilitating the study of the (epi)transcriptome at single molecule resolution.
Topics & Concepts
MinionComputer scienceNanopore sequencingWorkflowPipeline (software)RNASoftware portabilityOS XSoftwareData miningComputational biologyDNA sequencingDatabaseOperating systemDNABiologyGeneticsGeneRNA modifications and cancerRNA and protein synthesis mechanismsCancer-related molecular mechanisms research