LRP6 Is a Functional Receptor for Attenuated Canine Distemper Virus
Vaiva Gradauskaite, Marine Inglebert, John G. Doench, Melanie Scherer, Martina Dettwiler, Marianne Wyss, Neeta Shrestha, Sven Rottenberg, Philippe Plattet
Abstract
, is employed as a safe and efficient vaccine for dogs against distemper disease. Importantly, although CDV-OP can infect and kill multiple cancer cell lines, the basic mechanisms of entry remain to be elucidated, as most of those transformed cells do not express natural receptors (i.e., SLAM and nectin-4). In this study, using a genome-wide CRISPR/Cas9 knockout screen, we describe the discovery of LRP6 as a novel functional entry receptor for CDV-OP in various cancer cell lines and thereby uncover a basic mechanism of cell culture adaptation. Since LRP6 is upregulated in various cancer types, our data provide important insights in order to further investigate the oncolytic properties of CDV-OP.