Chemically synthesized circular RNAs with phosphoramidate linkages enable rolling circle translation
Kosuke Nakamoto, Naoko Abe, Genichiro Tsuji, Yasuaki Kimura, Fumiaki Tomoike, Yoshihiro Shimizu, Hiroshi Abe
Abstract
Circular RNA without a stop codon enables rolling circle translation. To produce circular RNAs, we carried out one-pot chemical synthesis of circular RNA from RNA fragments with the use of an EDC/HOBt-based chemical ligation reaction. The synthesized circular RNAs acted as translation templates, despite the presence of unnatural phosphoramidate linkages.
Topics & Concepts
PhosphoramidateTranslation (biology)Rolling circle replicationCircular RNARNATemplateChemistryStereochemistryNanotechnologyMessenger RNABiochemistryMaterials scienceDNAGenePolymeraseCircular RNAs in diseasesMXene and MAX Phase MaterialsSemiconductor materials and interfaces