Reusable and universal impedimetric sensing platform for the rapid and sensitive detection of pathogenic bacteria based on bacteria-imprinted polythiophene film
Lingling Wang, Xiaohui Lin, Ting Liu, Zhaohuan Zhang, Jie Kong, Hai Yu, Juan Yan, Donglei Luan, Yong Zhao, Xiaojun Bian
Abstract
from multi-bacterial strain mixtures. It also demonstrates applicability in the analysis of real lettuce and shrimp samples with good recoveries. Most significantly, the BIF sensing interface can be reused up to five times with good signal retention. Compared with most reported methods, this sensor is more rapid with a much shorter total assay time of 30 min, including the BIF preparation, bacterial rebinding, and impedance detection. This assay may hold great potential to help in the rapid, sensitive, and label-free detection of pathogenic bacteria in fields of food safety and public health.
Topics & Concepts
Detection limitAnalyteStaphylococcus aureusPolythiopheneBacteriaPathogenic bacteriaMolecular imprintingMaterials scienceChemistryChromatographyElectrodeMicrobiologyNanotechnologySelectivityBiologyConductive polymerOrganic chemistryGeneticsPhysical chemistryCatalysisBiosensors and Analytical DetectionAdvanced biosensing and bioanalysis techniquesAdvanced Chemical Sensor Technologies