Use of Clodronate Liposomes to Deplete Phagocytic Immune Cells in Drosophila melanogaster and Aedes aegypti
Jyothsna Ramesh Kumar, J. P. Downie Smith, Hyeogsun Kwon, Ryan C. Smith
Abstract
. Through microscopy and molecular techniques, we validate the depletion of phagocytic cell populations in both insect species and demonstrate the integral role of phagocytes in combating bacterial pathogens. Together, these data demonstrate the wide utility of CLD in insect systems to advance the study of phagocyte function in insect innate immunity.
Topics & Concepts
Immune systemBiologyInnate immune systemDrosophila melanogasterAedes aegyptiImmunityInsectAcquired immune systemPhagocyteCell biologyImmunologyEcologyLarvaGeneticsGeneInvertebrate Immune Response MechanismsInsect symbiosis and bacterial influencesMosquito-borne diseases and control