Pluripotent pig embryonic stem cell lines originating from in vitro-fertilized and parthenogenetic embryos
Kwang‐Hwan Choi, Dong‐Kyung Lee, Jong‐Nam Oh, Seung‐Hun Kim, Mingyun Lee, Sang‐Ho Woo, Dae-Yong Kim, Chang‐Kyu Lee
Abstract
Here, we report in vivo developmental competent pig embryonic stem cells (ESCs) originating from in vitro-fertilized and parthenogenetic embryos cultured in chemically defined culture media. The pig ESC lines expressed pluripotency markers and were capable of forming teratomas following subcutaneous injection into nude mice. These cell lines would be a useful resource for comparative developmental biology and agricultural biotechnology.
Topics & Concepts
BiologyParthenogenesisEmbryonic stem cellEmbryoInduced pluripotent stem cellIn vitroCell biologyCell cultureDevelopmental biologyEmbryoid bodyStem cellBlastocystIn vivoEmbryogenesisMolecular biologyGeneticsGenePluripotent Stem Cells ResearchCRISPR and Genetic EngineeringAnimal Genetics and Reproduction