Direct single-nucleotide resolution sequencing of DNA 5-methylcytosine using engineered DNA methyltransferase-mediated CMD-seq
Wei Liu, Zhaocheng Ma, Shan Zhang, Fang‐Yin Gang, Tong‐Tong Ji, Yao-Hua Gu, Neng‐Bin Xie, Shu-Yi Gu, Xia Guo, Feng Tian, Yu Liu, Jun Xiong, Bi‐Feng Yuan
Abstract
in human lung cancer tissue and adjacent normal tissue. The quantification results were highly comparable to those obtained using traditional bisulfite sequencing. Overall, CMD-seq provides a valuable tool for bisulfite-free, single-nucleotide resolution, and quantitative detection of 5mC in limited DNA samples.
Topics & Concepts
5-MethylcytosineMethyltransferaseDNA methyltransferaseDNANucleotideDNA sequencingChemistryDNA nanoball sequencingComputational biologyDNA methylationGeneticsBiologyBiochemistryMethylationBase sequenceGenomic libraryGeneGene expressionEpigenetics and DNA MethylationRNA modifications and cancer