A Dual Endogenous Stimuli-Responsive Framework Nucleic Acid Nanodevice for MicroRNA Imaging
Lie Li, Haiyan Chen, Wen Xiaohong, Suping Li, Mei Yang, Qiuping Guo, Kemin Wang
Abstract
Open AccessCCS ChemistryRESEARCH ARTICLES29 Dec 2022A Dual Endogenous Stimuli-Responsive Framework Nucleic Acid Nanodevice for MicroRNA Imaging Lie Li, Haiyan Chen, Xiaohong Wen, Suping Li, Mei Yang, Qiuping Guo and Kemin Wang Lie Li , Haiyan Chen , Xiaohong Wen , Suping Li , Mei Yang , Qiuping Guo and Kemin Wang https://doi.org/10.31635/ccschem.022.202202465 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareFacebookTwitterLinked InEmail Framework nucleic acids (FNAs) have emerged as intelligent sensing systems for the detection of tumor-related biomarkers in living cells. However, orthogonally controlled manipulation of FNAs-based nanodevices for on-site imaging of microRNAs (miRNAs) remains an intractable challenge. Herein, we report a dual endogenous stimuli-responsive framework nucleic acid nanodevice that can perform miRNA sensing and imaging in a tumor-specific manner. The sensing function of the nanodevice is silent (OFF) by rationally incorporated ATP aptamer and abasic site, which can be activated (ON) with ATP and human apurinic/apyrimidinic endonuclease 1 (APE1) in tumor cells, enabling the on-site and efficient miRNA imaging with improved tumor specificity. Furthermore, we demonstrate the capability of the nanodevice for specific miRNA imaging both in living cells and in vivo based on the "dual keys" (overexpressed ATP and APE1 in tumors) priming mode. Therefore, this work illustrates a simple biosensing methodology with great potential for precise imaging of tumor biomarkers in clinical diagnosis and therapeutic evaluation. Download figure Download PowerPoint Previous articleNext article FiguresReferencesRelatedDetails Issue AssignmentVolume 0Issue jaPage: 1-19 Copyright & Permissions© 2022 Chinese Chemical Society Downloaded 0 times PDF downloadLoading ...