Nano illumination microscopy: a technique based on scanning with an array of individually addressable nanoLEDs
Nil Franch, Joan Canals, Victor Moro, A. Vilà, A. Romano‐Rodrı́guez, Juan Daniel Prades, Jan Gülink, Daria Bezshlyakh, A. Waag, Katarzyna Kluczyk-Korch, Matthias Auf der Maur, Aldo Di Carlo, Á. Diéguez
Abstract
In lensless microscopy, spatial resolution is usually provided by the pixel density of current digital cameras, which are reaching a hard-to-surpass pixel size / resolution limit over 1 µm. As an alternative, the dependence of the resolving power can be moved from the detector to the light sources, offering a new kind of lensless microscopy setups. The use of continuously scaled-down Light-Emitting Diode (LED) arrays to scan the sample allows resolutions on order of the LED size, giving rise to compact and low-cost microscopes without mechanical scanners or optical accessories. In this paper, we present the operation principle of this new approach to lensless microscopy, with simulations that demonstrate the possibility to use it for super-resolution, as well as a first prototype. This proof-of-concept setup integrates an 8 × 8 array of LEDs, each 5 × 5 μm 2 pixel size and 10 μm pitch, and an optical detector. We characterize the system using Electron-Beam Lithography (EBL) pattern. Our prototype validates the imaging principle and opens the way to improve resolution by further miniaturizing the light sources.