Integration of single-molecule detection with endonuclease IV-assisted signal amplification for sensitive DNA methylation assay
Yan Zhang, Jinping Hu, Xiaoran Zou, Fei Ma, Jian‐Ge Qiu, Chun‐yang Zhang
Abstract
We develop a new fluorescent biosensor for sensitive DNA methylation assay by integrating single-molecule detection with endo IV-assisted signal amplification.
Topics & Concepts
EndonucleaseDNAMolecular biologyChemistryDNA methylationBiosensorMethylationFluorescenceSingle molecule real time sequencingRestriction enzymeMoleculeBiophysicsBiologyBiochemistryGeneDNA sequencingDNA sequencerPhysicsGene expressionQuantum mechanicsOrganic chemistryAdvanced biosensing and bioanalysis techniquesEpigenetics and DNA MethylationSingle-cell and spatial transcriptomics