Cell-free hemoglobin triggers macrophage cytokine production via TLR4 and MyD88
Kaitlyn Schaaf, Stuart R. Landstreet, Sangami Pugazenthi, Emily Y. Qian, Nathan D. Putz, Tatiana Siderova, Allison M. Owen, Julia K. Bohannon, Lorraine B. Ware, Julie A. Bastarache, Ciara M. Shaver
2023American Journal of Physiology-Lung Cellular and Molecular Physiology15 citationsDOIOpen Access PDF
Abstract
Cell-free hemoglobin (CFH) is elevated in the airspace of most patients with acute respiratory distress syndrome and causes severe inflammation. Here, we identify that CFH contributes to macrophage-induced cytokine production via Toll-like receptor 4 (TLR4) and myeloid differentiation primary response 88 (MyD88) signaling. These data increase our knowledge of the mechanisms through which CFH contributes to lung injury and may inform development of targeted therapeutics to attenuate inflammation.
Topics & Concepts
TLR4Proinflammatory cytokineBronchoalveolar lavageChemokineImmunologyTumor necrosis factor alphaCytokineCXCL1Interleukin 10InflammationChemistryARDSAlveolar macrophageLungMedicineMacrophageIn vitroInternal medicineBiochemistryRespiratory Support and MechanismsNeonatal Respiratory Health ResearchImmune Response and Inflammation