Evidence for the Chemical Mechanism of RibB (3,4-Dihydroxy-2-butanone 4-phosphate Synthase) of Riboflavin Biosynthesis
Nikola Kenjić, Kathleen M. Meneely, Daniel Wherritt, Melissa C. Denler, Timothy A. Jackson, Graham R. Moran, Audrey L. Lamb
Abstract
soaks of VcRibB-d-Ru5P crystals show accumulation in crystallo of the same intermediates. Collectively, these data reveal for the first time crucial transient chemical states in the mechanism of RibB.
Topics & Concepts
ChemistryFormateStereochemistrySubstrate (aquarium)BiosynthesisEnzymeATP synthaseActive siteCatalysisOrganic chemistryGeologyOceanographyEnzyme Structure and FunctionMicrobial Metabolic Engineering and BioproductionAmino Acid Enzymes and Metabolism