Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq
Vincent Hahaut, Dinko Pavlinić, Walter Carbone, Sven Schuierer, Pierre Balmer, Mathieu Quinodoz, Magdalena Renner, Guglielmo Roma, Cameron S. Cowan, Simone Picelli
Abstract
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.
Topics & Concepts
Flash (photography)RNA-SeqIdentifierComputer scienceComputational biologyProtocol (science)BiologyGeneGene expressionGeneticsTranscriptomePhysicsMedicineComputer networkOpticsPathologyAlternative medicineSingle-cell and spatial transcriptomicsExtracellular vesicles in diseaseCancer-related molecular mechanisms research