Production of Recombinant Replication-defective Lentiviruses Bearing the SARS-CoV or SARS-CoV-2 Attachment Spike Glycoprotein and Their Application in Receptor Tropism and Neutralisation Assays
Nazia Thakur, Giulia Gallo, Ahmed Elreafey, Dalan Bailey
Abstract
proteins, for the reconstitution of lentiviral particles, and a plasmid coding for the viral attachment protein of interest. This approach allows the investigation of different aspects of viral entry, such as the identification of receptor tropism, the prediction of virus host range, and zoonotic transmission potential, as well as the characterisation of antibodies (sera or monoclonal antibodies) and pharmacological inhibitors that can block entry. Graphic abstract: SARS-CoV and SARS-CoV-2 pseudoparticle generation and applications.
Topics & Concepts
TropismRecombinant DNAVirologyViral entryGlycoproteinPlasmidMonoclonal antibodyNeutralizationBiologyTransfectionLentivirusViral envelopeVirusPolyproteinsBaculoviridaeCell cultureViral vectorAntibodyTissue tropismLyssavirusRecombinant virusCell biologyXenotransplantationReceptorHEK 293 cellsChemistryMolecular biologySmall interfering RNACapsidViral InterferenceNeutralizing antibodyRNATransduction (biophysics)Phage displaySARS-CoV-2 and COVID-19 ResearchVirus-based gene therapy researchAnimal Virus Infections Studies