Characterization of βARKct engineered cellular extracellular vesicles and model specific cardioprotection
Jin‐Sook Kwon, Sarah M. Schumacher, Erhe Gao, J. Kurt Chuprun, Jessica Ibetti, Rajika Roy, Mohsin Khan, Raj Kishore, Walter J. Koch
Abstract
βARKct, the peptide inhibitor of GRK2, improves survival and metabolic functions of cardiac-derived progenitor cells. As any benefit of stem cells in the ischemic and injured heart suggests paracrine mechanisms via secreted EVs, we investigated whether CDC-βARKct engineered EVs would show any benefit over control CDC-EVs. Compared with control EVs, βARKct-containing EVs displayed some unique beneficial properties that may be due to altered pro- and anti-inflammatory cytokines within the vesicles.
Topics & Concepts
Paracrine signallingCardioprotectionCell biologyExtracellular vesiclesProgenitor cellChemistryMicrovesiclesStem cellBiologyMedicineBiochemistrymicroRNAMyocardial infarctionInternal medicineGeneReceptorExtracellular vesicles in diseaseRNA Interference and Gene DeliveryMicroRNA in disease regulation