Carbohydrate Metabolism Affects Macrophage-Mediated Killing of Enterococcus faecalis
Lifan Wei, Feng Xia, Jia Wang, Shujun Ran, Yakun Liang, Wei Zhou, Zhengwei Huang, Jingping Liang
Abstract
E. faecalis has become a major pathogen leading to a variety of infections around the world. The metabolic interaction between E. faecalis and its host is important during infection but is rarely investigated. We used transposon insertion sequencing coupled with transcriptome sequencing to explore the metabolic interaction between E. faecalis and macrophages and uncovered that the shift of carbohydrate metabolism dramatically affected the inflammatory response of macrophages. In addition, E. faecalis attenuated carbohydrate metabolism to avoid the activation of the immune response of macrophages. This study provides new insights for the reason why E. faecalis is capable of long-term survival in macrophages and may facilitate the development of novel strategies to treat infectious diseases.