Enabling highly ( <i>R</i> )-enantioselective epoxidation of styrene by engineering unique non-natural P450 peroxygenases
Panxia Zhao, Jie Chen, Nana Ma, Jingfei Chen, Xiangquan Qin, Chuanfei Liu, Fuquan Yao, Lishan Yao, Long Yi Jin, Zhiqi Cong
Abstract
)-styrene oxide performed at 0 °C with high conversion, maintaining enantioselectivity, and moderate isolated yields, further suggests the potential application of the current P450 enzymatic system in styrene epoxidation. This study indicates that the synergistic use of protein engineering and an exogenous DFSM constitutes an efficient strategy to control the enantioselectivity of styrene epoxidation, thus substantially expanding the chemical scope of P450 enzymes as useful bio-oxidative catalysts.
Topics & Concepts
Enantioselective synthesisStyreneMonooxygenaseChemistryMoleculeProtein engineeringDual (grammatical number)Cytochrome P450Combinatorial chemistryOrganic chemistryStereochemistryCatalysisEnzymeCopolymerPolymerArtLiteraturePharmacogenetics and Drug MetabolismMetal-Catalyzed Oxygenation MechanismsSynthesis and Catalytic Reactions