Computational Photon Counting Using Multithreshold Peak Detection for Fast Fluorescence Lifetime Imaging Microscopy
Janet E. Sorrells, Rishyashring R. Iyer, Lingxiao Yang, Elisabeth Moy Martin, Geng Wang, Haohua Tu, Marina Marjanović, Stephen A. Boppart
Abstract
and a human breast cancer cell line undergoing apoptosis that this novel multithreshold peak detection method can accurately characterize the intensity and fluorescence lifetime of samples producing photon rates up to 223%, higher than previously demonstrated photon counting FLIM systems.
Topics & Concepts
Photon countingPhotomultiplierDetectorOpticsPhotonPhotodetectorFluorescence-lifetime imaging microscopyPhysicsMicroscopyDead timeFluorescenceSilicon photomultiplierMaterials scienceScintillatorQuantum mechanicsAdvanced Fluorescence Microscopy TechniquesAdvanced Optical Sensing TechnologiesCell Image Analysis Techniques