The Stringent Response Inhibits 70S Ribosome Formation in <i>Staphylococcus aureus</i> by Impeding GTPase-Ribosome Interactions
Daniel J. Bennison, Jose A. Nakamoto, Timothy D. Craggs, Pohl Milón, John B. Rafferty, Rebecca M. Corrigan
Abstract
and within bacterial cells, with the ppGpp-bound state structurally mimicking the inactive GDP-bound conformation of the enzyme. We additionally reveal that these GTPase enzymes have a greater affinity for OFF-state-inducing nucleotides, which is a mechanism likely to control ribosome assembly during growth. With this, we further our understanding of how ribosome function is controlled by (p)ppGpp, enabling bacterial survival during stress.
Topics & Concepts
Stringent responseGTPaseRibosomeCell biologyEffectorNucleotideRibosome biogenesisGuanosine triphosphateChemistryBiochemistryBiologyRibosomal proteinGTP'Ribosomal RNAEukaryotic RibosomeProtein biosynthesisTranslation (biology)A-siteBiogenesisInitiation factorInternal ribosome entry siteEnzyme50SGTP-binding protein regulatorsBacterial Genetics and BiotechnologyRNA and protein synthesis mechanismsRNA modifications and cancer