Multilayered VBC score predicts sgRNAs that efficiently generate loss-of-function alleles
Georg Michlits, Julian Jude, Matthias Hinterndorfer, Melanie de Almeida, Gintautas Vainorius, Maria Hubmann, Tobias Neumann, Alexander Schleiffer, Thomas R. Burkard, Michaela Fellner, Max Gijsbertsen, Anna K. Traunbauer, Johannes Zuber, Ulrich Elling
Topics & Concepts
CRISPRFrameshift mutationIndelBiologySelection (genetic algorithm)Computational biologyGeneticsCas9Function (biology)GeneLoss functionGenome editingAlleleMutationComputer sciencePhenotypeSingle-nucleotide polymorphismMachine learningGenotypeCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanisms