Litcius/Paper detail

Diagnosis and Treatment of Bacterial Pneumonia in Critically Ill Patients with COVID-19 Using a Multiplex PCR Assay: A Large Italian Hospital’s Five-Month Experience

Brunella Posteraro, Venere Cortazzo, Flora Marzia Liotti, Giulia Menchinelli, Chiara Ippoliti, Giulia De Angelis, Marilena La Sorda, Gennaro Capalbo, Joel Vargas, Massimo Antonelli, Maurizio Sanguinetti, Gennaro De Pascale, Teresa Spanu

2021Microbiology Spectrum31 citationsDOIOpen Access PDF

Abstract

Since bacterial pneumonia is relatively frequent, suspicion of it in COVID-19 patients may prompt ICU clinicians to overuse (broad-spectrum) antibiotics, particularly when empirical antibiotics do not cover the suspected pathogen. We showed that a PCR-based, culture-independent laboratory assay allows not only accurate diagnosis but also streamlining of antimicrobial therapy for bacterial pneumonia episodes. We report on the actual implementation of rapid diagnostics and its real-life impact on patient treatment, which is a gain over previously published studies on the topic. A better understanding of the role of that or similar PCR assays in routine ICU practice may lead us to appreciate the effectiveness of their implementation during the COVID-19 pandemic.

Topics & Concepts

MedicinePneumoniaAcinetobacter baumanniiAntimicrobialAntibioticsBacterial pneumoniaPseudomonas aeruginosaStaphylococcus aureusInternal medicineIntensive care unitAntibiotic resistanceVentilator-associated pneumoniaMicrobiological cultureMicrobiologyMultiplex polymerase chain reactionDrug resistanceIntensive care medicineAcinetobacterIntensive careAntibacterial agentRespiratory diseaseEtiologyStaphylococcusGold standard (test)Antibiotic Use and ResistanceCOVID-19 Clinical Research StudiesAntibiotics Pharmacokinetics and Efficacy