An ultrasensitive biosensing platform for FEN1 activity detection based on target-induced primer extension to trigger the collateral cleavage of CRISPR/Cas12a
Xiaoying Cai, Dan Zhao, Xingrong Li, Qingyuan Zheng, Xiaojia Shu, Shijia Ding, Decai Zhang, Yurong Yan
Topics & Concepts
ChemistryEndonucleaseDNADNA polymeraseDetection limitPrimer extensionPrimer (cosmetics)BiosensorMolecular biologyCleavage (geology)Rolling circle replicationBiophysicsComputational biologyBiochemistryChromatographyBiologyPaleontologyFracture (geology)Base sequenceOrganic chemistryCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesDNA Repair Mechanisms