Elucidation of metabolic pathways of 25-hydroxyvitamin D3 mediated by CYP24A1 and CYP3A using Cyp24a1 knockout rats generated by CRISPR/Cas9 system
Kaori Yasuda, Miyu Nishikawa, Kairi Okamoto, Kyohei Horibe, Hiroki Mano, Mana Yamaguchi, Risa Okon, Kimie Nakagawa, Naoko Tsugawa, Toshio Okano, Fumihiro Kawagoe, Atsushi Kittaka, Shinichi Ikushiro, Toshiyuki Sakaki
Abstract
D3 into 25(OH)D3-26,23-lactone in both rats and humans. Taken together, our data indicate that Cyp24a1 KO rats are valuable for metabolic studies of vitamin D and its analogs. In addition, long-term administration of 25(OH)D3 to Cyp24a1 KO rats at 110 μg/kg body weight/day resulted in significant weight loss and ectopic calcification. Thus, Cyp24a1 KO rats could represent an important model for studying renal diseases originating from CYP24A1 dysfunction.
Topics & Concepts
CRISPRCYP24A1Cas9Computational biologyBiologyChemistryVitamin D and neurologyGeneticsGeneEndocrinologyCalcitriol receptorVitamin D Research StudiesHormonal Regulation and HypertensionAdrenal Hormones and Disorders